Sequencing Grade Chymotrypsin for Digestion Reactions In-solution
Sequencing Grade Chymotrypsin for Digestion Reactions In-solution
Sequencing Grade Chymotrypsin for Digestion Reactions In-solution
Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

MOQ:100ug
Price:158$/100ug
PRODUCT DESCRIPTION
High Light:

sequencing grade modified trypsin

,

serine protease

Brand Name YaxinBio
Certification ISO9001
Delivery Time 7 days
Minimum Order Quantity 100ug
View More
ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
View More
Get the Best Price for
Sequencing Grade Chymotrypsin for Digestion Reactions In-solution
Sequencing Grade Chymotrypsin for Digestion Reactions In-solution
MOQ: 100ug
Price:158$/100ug
Continue
Recommended Products
About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

Get Latest Price For Your Requirement
PRODUCT DESCRIPTION
High Light:

sequencing grade modified trypsin

,

serine protease

Place of Origin China
Brand Name YaxinBio
Certification ISO9001
Model Number SRCT03
Minimum Order Quantity 100ug
Price 158$/100ug
Packaging Details dry ice
Delivery Time 7 days
Payment Terms T/T
Supply Ability 1000g per week
Source E.Coli
Purity NLT 95% by HPLC analysis.
Physical form Lyophilized powder
Specific activity NLT 1500 USP units/mg pro
Contaminant activity No carboxypeptidase A, or other proteases contaminant.
CAS 9004-07-3

 

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

Description:

Sequencing Grade Chymotrypsin for Digestion Reactions In-solution

 

 

Descirption

 

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.

The sequencing grade human chymotrypsin can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

CAS: 9004-07-3
EC: 3.4.21.1

Source: human chymotrypsin, expressed in E. Coli.

 

Main Features

 

Source

E. Coli

M.W.

26,950 Da  

Purity

≥ 98 % by HPLC.

Format

Lyophilized

Specific activity

≥ 3000 units/mg pro

 

Application

 

1) Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

2) This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

 

Recommend Usage

 

For peptide digestion, use a ratio (w/w) of approximately 1:20-1:100 for chymotrypsin:peptide. Perform peptide digests in 40 mM Tris-HCl pH7.8 containing 5 mM CaCl2, at 30 °C. Self digestion may occur if temperatures above 37°C.

 

Storage Instructions

 

Recombinant Chymotrypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

Get Latest Price For Your Requirement