Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery

Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery

MOQ:100U
Price:65$/100u
PRODUCT DESCRIPTION
High Light:

bovine enterokinase

,

recombinant enzymes

Brand Name YaxinBio
Certification NSF ISO 9001:2015
Minimum Order Quantity 100U
Model Number REK08
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery
MOQ: 100U
Price:65$/100u
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

Get Latest Price For Your Requirement
PRODUCT DESCRIPTION
High Light:

bovine enterokinase

,

recombinant enzymes

Place of Origin China
Brand Name YaxinBio
Certification NSF ISO 9001:2015
Model Number REK08
Minimum Order Quantity 100U
Price 65$/100u
Packaging Details 100U, 500U, 1000U, 10KU, 100KU, 1MU; ice packaging, carton
Payment Terms T/T
Supply Ability 1000ku per week
Source E. Coli
M.W. Theoretical MW: 25,850 Da;The apparent MW on SDS-PAGE: about 27,000 Da
Storage Store at -20°C after delivery.
Specific activity One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in a buffer 25mMTris-HCl, pH 8.0.Substrate: a special fusion protein.
Stability Keep cool with blue ice during shipping.Remained stable at 25°C for one week without activity lost. Can be stable after several times of freeze-thaw.
Cat.No REK08

 

Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery

Description:

Recombinant Enterokinase, Expressed in E.coli, Stored under -20°C after delivery

 

Recombinant Enterokinase

 

Catalog Number:REK08

CAS:9017-74-8
EC:3.4.21.9

Storage temperature: -20°C or below

Source: Recombinant enterokinase is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant enterokinase is identical to bovine enterokinase light chain

 

1.Description

 

Enterokinase is a serine proteinase in the duodenum that plays a critical role in mammalian digestion. It converts trypsinogen into its active form trypsin, by cleaving its aminoterminal hexapeptide Val(Asp)4-Lys. More recently, the enterokinase has been shown to have a broad utility in cleaving fusion proteins produced in Escherichia coli. The enzyme is particularly suitable for this role because of its high degree of specificity, its tolerance to a wide range of reaction conditions, and the fact that its recognition sequence lies entirely on the aminoterminal side of the scissile bond. This enzymatic activity allows release of carboxyl-terminal fusion partners from fusion proteins without leaving unwanted amino acid residues on their amino termini.

 

 

2.MAIN FEATURES

 

Source Recombinant E. coli
Appearance Clear,colorless to yellowish liquid
Activity ≥5 u/μl
Purity(SDS-PAGE) Single major band
Molecular Weight(SDS-PAGE) 25.8 ±2.6 kDa

UNIT DEFINITION:One unit is defined as the amount of enzyme needed to cleave 0.5mg of fusion protein in 12 to16 hours to get 95% completion at 25°C in 25mMTris-HCl, pH 8.0. Substrate: a special fusion protein.

 

3.Advantages

  1. Animal origin free: Recombinant enterokinase is no exogenous virus contamination,and any animal origin material is not used in the production process.
  2. Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
  3. High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
  4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
  5. Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
  6. Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

4. STABILITY OF STORAGE AND TRANSPORT

(1)Stability of storage: Recombinant enterokinase should be stored under -20°C in sealed container.It is stable within 24 months.If stored at 25°C ,it is stable within one week.It is no activity loss after 5 times repeated freezing and thawing.

(2)Stability of transport: The product is stable by blue ice insulation transport.

 


 

 

 

 

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