Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli
Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli
Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli
Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli

Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli

MOQ:100ug
Price:98$/100ug
PRODUCT DESCRIPTION
High Light:

trypsin endopeptidase

,

serine protease

Brand Name YaxinBio
Certification ISO 9001 2015
Delivery Time 7 days
Minimum Order Quantity 100ug
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli
Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli
MOQ: 100ug
Price:98$/100ug
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

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PRODUCT DESCRIPTION
High Light:

trypsin endopeptidase

,

serine protease

Place of Origin China
Brand Name YaxinBio
Certification ISO 9001 2015
Model Number SRT0201
Minimum Order Quantity 100ug
Price 98$/100ug
Packaging Details ice packaging
Delivery Time 7 days
Payment Terms T/T
Supply Ability 1000g per week
Source E. Coli
Purified by HPLC
Physical form Lyophilized powder
Specific activity >4500 USP units/mg pro
Purity One single main band at 24kD by SDS-PAGE
Contaminant activity No chymotrypsin, carboxypeptidase A, or other protease contaminant.

 

Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli

Description:

Sequencing Grade Trypsin, Purified by HPLC and Expressed in E.coli

 

Sequencing Grade Trypsin

 

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

Main Features

 

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade trypsin lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃.

It is no activity loss after 5 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

Reconstitution

 

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

 

Specificity

 

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

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