Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Enzyme Markers Monoclonal and Polyclonal Antibody Purification

Enzyme Markers Monoclonal and Polyclonal Antibody Purification

MOQ:10mg
Price:79$/10mg
PRODUCT DESCRIPTION
High Light:

protein A column

,

purified protein

Brand Name YaxinBio
Certification ISO 9001 2015
Delivery Time 5-8 weekdays
Minimum Order Quantity 10mg
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Enzyme Markers Monoclonal and Polyclonal Antibody Purification
Enzyme Markers Monoclonal and Polyclonal Antibody Purification
MOQ: 10mg
Price:79$/10mg
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

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PRODUCT DESCRIPTION
High Light:

protein A column

,

purified protein

Place of Origin China
Brand Name YaxinBio
Certification ISO 9001 2015
Model Number RSPA05
Minimum Order Quantity 10mg
Price 79$/10mg
Packaging Details ice packaging, carton
Delivery Time 5-8 weekdays
Payment Terms T/T
Supply Ability 1000g per week
Substrate Porous polystyrene-divinylbenzene
Particle size 40 um
Ligand Recombinant Protein A
Recommend flow rate 300-700 cm/h
DBC At least 300 mg IgG/mL media at 500 cm/h
Maximum pressure 1500 psi
brand yaxinbio

 

Enzyme Markers Monoclonal and Polyclonal Antibody Purification

Description:

Enzyme Markers Monoclonal and Polyclonal Antibody Purification

 

Recombinant Protein A

 

Catalog Number:RSPA05

CAS:91932-65-9

Storage temperature: 2-8℃

Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli

 

1.DESCRIPTION

Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.

 

2.MAIN FEATURES

Appearance White or off white powder
Solubility Clear liquid
Bioburden ≤ 1 cfu/ mg
SDS-PAGE Single major band
SDS-PAGE (MW) 25000±1000 Daltons
UV Spectrum E275/250 ≥ 1.2
E0.1% at 275nm = 0.17-0.23
Purity (HPLC) ≥ 95%

 

3.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.

After dissolved with pure water, it should be stored under -20℃.

Stability of transport: The product is stable by blue ice insulation transport.

 

 

4.APPLICATION:

The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

 

5.Product Specification

Test/Method                    

Specification

Bioburden

No organisms detected.

12% SDS-PAGE

Single major band

Coomassie Stain

25kD

UV Spectrum

E275/250 ≥1.2,

E0.1% at 275nm=0.17-0.23 

UV spectrometry

No DNA or RNA detected

Triton Content

No Triton contaminant.

Format

Lyophilized

pH stable range

pH 1-1

 

 

 

 

 

 

 

 

 

 

 

 

 

6.Storage Instructions

Recommend recombinant trypsin lyophilized should be stored under 2-8℃in sealed container.It is stable within 24 months.

 

7.Usage

For Research or Manufacturing Purpose Only. Not for Human.

 

 

8.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase

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