Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

MOQ:10mg
Price:65$/10mg
PRODUCT DESCRIPTION
High Light:

porcine trypsin

,

trypsin enzyme

Brand Name YaxinBio
Certification NSF ISO 9001:2015
Delivery Time 6 days
Minimum Order Quantity 10mg
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
MOQ: 10mg
Price:65$/10mg
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

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PRODUCT DESCRIPTION
High Light:

porcine trypsin

,

trypsin enzyme

Place of Origin China
Brand Name YaxinBio
Certification NSF ISO 9001:2015
Model Number RPT0201
Minimum Order Quantity 10mg
Price 65$/10mg
Packaging Details ice packaging, carton
Delivery Time 6 days
Payment Terms T/T
Supply Ability 1000g per week
Product Recombinant Porcine Trypsin
Cat. No. RPT0201
Physical form White or White-like lyophilized powder
Additives Carbohydrates
Protein content 35% ~ 70%
Related Product Recombinant Trypsin Solution

 

Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin

Description:

Recombinant Trypsin, For Cell Culture, Protein Digestion, Dissociate Tissue Cells, Trypsin
 
Recombinant Trypsin
Description
 
Trypsin
Enzyme Commission (EC) Number: 3.4.21.4
Molecular Weight: 23.3 kDa (porcine)
Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
pI: 10.1 - 10.5(porcine)

Trypsinogen
Molecular Weight: 24 kDa (porcine)
Extinction Coefficient: E1% = 14.4 (280 nm)
pI: 9.3 (porcine)

Procedure

Pipette (in milliliters) the following reagents into suitable quartz cuvettes:

 TestBlank
Reagent B (BAEE)3.003.00

 

 

 

Equilibrate to 25 °C. Monitor the A253nm until constant, using a suitably thermostatted spectrophotometer. Then add:

 TestBlank
Reagent C (HCl)----0.20
Reagent D (Enzyme Solution)0.20----

 

 

 

 

Immediately mix by inversion and record the increase in A253nm for approximately 5 minutes. Obtain the ΔA253nm/minute using the maximum linear rate for both the Test and Blank.

 

Calculation

 

BAEE Units/ml enzyme =(ΔA253nm/min Test - ΔA253nm/min Blank)(df)
(0.001) (0.20)

 

 

 

df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction mix
0.20 = Volume (in milliliters) of enzyme used

Units/mg solid=units/ml enzyme
mg solid/ml enzyme

 
 
 
Advantages
 
Animal origin free:The use of recombinant Porcine Trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. 
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
 
Application
Use Trypsin recombinant, Proteomics Grade, to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.
 
Recommend Usage
 
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7-10.
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