Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

MOQ:10mg
Price:89$/10mg
PRODUCT DESCRIPTION
High Light:

trypsin digestion

,

stem cell culture

Brand Name YaxinBio
Certification ISO 9001 2015
Minimum Order Quantity 10mg
Model Number RHT03
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin
MOQ: 10mg
Price:89$/10mg
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

Get Latest Price For Your Requirement
PRODUCT DESCRIPTION
High Light:

trypsin digestion

,

stem cell culture

Place of Origin China
Brand Name YaxinBio
Certification ISO 9001 2015
Model Number RHT03
Minimum Order Quantity 10mg
Price 89$/10mg
Packaging Details ice packaging, carton
Payment Terms T/T
Supply Ability 1000g per week
Product Recombinant Human Trypsin 2
Packaging 10mg,100mg or bulk
Specific activity ≥2500 USP u/mg pro.
Contaminant activity No chymotrypsin, carboxypeptidase A, and other protease contaminant.
Related Product Recombinant Porcine Trypsin
Manufacturer YaxinBio

 

Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

Description:

Tissue Cells Dissociation, Protein Degradation, Digest Protein, Recombinant Human Trypsin

 

Recombinant Human Trypsin

 

Description

 

1.Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.
2.Recombinant human trypsin is  protein expressed in E.coli through a genetically engineered and purified by high pressure liquid chromatography.  Enzyme activities such as carboxypeptidase A and chymotrypsin are not contained in the trypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

 

 

 

 

 

 

 

 

MAIN FEATURES

Source Recombinant E. coli
Appearance White or off white powder
Specific activity ≥2500 USP units/mg pro.
Protein Content ≥ 90%
Purity(HPLC) ≥ 95%

UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

 

Advantages

Animal origin free:The use of recombinant human trypsin eliminates the risk of virus presence and any other potential adventitious agents found in animal pancreas-derived trypsin. YaxinBio recombinant human trypsin belongs to the AOF level 3.

Recombinant human trypsin:The amino acid sequence is the same as the human trypsin 2.

Stable:A sterile recombinant human trypsin lyophilized eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

Recombinant human trypsin provides increased specificity and eliminates

contaminating activities found in lower purity enzymes.

No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
Purity is more than 95% by HPLC.

 

 

 

 

 

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