Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC
Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC
Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC
Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC
Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC

Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC

MOQ:1mg
Price:1020$/g
PRODUCT DESCRIPTION
High Light:

stem cell culture

,

trypsinized cells

Brand Name YaxinBio
Certification ISO 9001 2015
Delivery Time 7 Days
Minimum Order Quantity 1mg
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC
Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC
MOQ: 1mg
Price:1020$/g
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

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PRODUCT DESCRIPTION
High Light:

stem cell culture

,

trypsinized cells

Place of Origin China
Brand Name YaxinBio
Certification ISO 9001 2015
Model Number RHT03
Minimum Order Quantity 1mg
Price 1020$/g
Packaging Details ice packaging, carton
Delivery Time 7 Days
Payment Terms T/T
Supply Ability 1000g per week
Product Recombinant Human Trypsin
Packaging 10mg,100mg or bulk
Source   E. Coli
Purified by HPLC
Related Product Recombinant Porcine Trypsin
Manufacturer YaxinBio

 

Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC

Description:

Stable Recombinant Human Trypsin Enzyme, Expressed in E.Coli and Purified by HPLC

 

Human Trypsin

Description

 

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end

of lysine and arginine amino acid residues. The optimum pH is pH 7 - 10. The enzyme is inhibited

by serine protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA.

Recombinant Human Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

 

Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen . Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas

and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes . Mutations in the PRSS1 gene can

cause hereditary pancreatitis

 

Main Features

 

Format

White or White-like lyophilized

Specific activity

≥ 2500 USP u/mg pro.

Purity

≥ 95% by HPLC

Contaminant activity

No chymotrypsin, carboxypeptidase A, and other protease contaminant.

 

 

 

 

 

 

 

Unit Definition

 

One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in

a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Storage Instructions

 

Recombinant human trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is

stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months

and above 90% activity remained after 10 times repeated freezing and thawing.

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