Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

MOQ:1mg
Price:385$/mg
PRODUCT DESCRIPTION
High Light:

trypsin endopeptidase

,

serine protease

Brand Name YaxinBio
Certification ISO 9001:2015
Minimum Order Quantity 1mg
Model Number SRT0201
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics
MOQ: 1mg
Price:385$/mg
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

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PRODUCT DESCRIPTION
High Light:

trypsin endopeptidase

,

serine protease

Place of Origin China
Brand Name YaxinBio
Certification ISO 9001:2015
Model Number SRT0201
Minimum Order Quantity 1mg
Price 385$/mg
Packaging Details ice packaging, carton
Payment Terms T/T
Supply Ability 1000g per week
Product Sequencing Grade Trypsin
Source E. Coli
Physical form Lyophilized powder
Specific activity >4500 USP units/mg pro
Purity One single main band at 24kD by SDS-PAGE
RELATED PRODUCT Recombinant Trypsin

 

Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

Description:

Sequencing Grade Recombinant Trypsin, Mass Spectrometry Grade For Proteomics

 

Sequencing Grade trypsin

 

Catalog Number:SRT0202

CAS:9002-07-7
EC:3.4.21.4

Source:Expressed in E. Coli, methylation modified.

 

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

 

Application

 

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

 

General description

 

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

 

PRODUCT USAGE

 

The amino acid sequence of sequencing grade recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin, and there are many advantages, such as no enzyme activity, high stability, non-self-cutting, high activity.When used, it is no non-specific fragments and self-cutting fragments.Sequencing grade recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as specific protein digestion, protein sequencing, peptide mapping, proteomics studies, Z-D peptides on the peptidase digestion.

 

Preparation Note

 

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

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