Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

MOQ:100ug
Price:198$/100ug
PRODUCT DESCRIPTION
High Light:

sequencing grade modified trypsin

,

serine protease

Brand Name YaxinBio
Certification NSF ISO 9001 2015
Minimum Order Quantity 100ug
Model Number SRT0201
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin
MOQ: 100ug
Price:198$/100ug
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

Get Latest Price For Your Requirement
PRODUCT DESCRIPTION
High Light:

sequencing grade modified trypsin

,

serine protease

Place of Origin China
Brand Name YaxinBio
Certification NSF ISO 9001 2015
Model Number SRT0201
Minimum Order Quantity 100ug
Price 198$/100ug
Packaging Details ice packaging, carton
Payment Terms T/T
Supply Ability 1000gper week
Product Sequencing Grade Trypsin
Cat. No. SRT0201
Packaging 100ug 1mg
Manufacturer YaxinBio
Purity One single main band at 24kD by SDS-PAGE
Contaminant activity No chymotrypsin, carboxypeptidase A, or other protease contaminant.

 

Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

Description:

Sequnencing Grade Trypsin For In-gel Protein Digestion, Sequencing Grade Modified Recombinant Trypsin

 

Sequencing Grade Trypsin

Description

 

Trypsin specifically hydrolyzes peptide bonds at the carboxyl side of lysine and arginine residues. Recombinant trypsin is free of any other proteases activities, and TPCK is unnecessary and not contained. Unmodified trypsin is subject to auto-proteolysis, generating fragments that can interfere with protein sequencing or HPLC/MS peptides analysis. YaxinBio’s sequencing grade modified trypsin is recombinant porcine trypsin modified by reductive methylation, rendering it resistant to proteolytic digestion.

 

Main Features

 

Source

E. Coli

Format

Liquid in 50mM HAc, or fluffy solid.

Protein concentration

0.5mg/ml in 50mM HAc

Specific activity

≥ 4500 USP units/mg pro

Purity(RP-HPLC)

≥99% by HPLC

Contaminant activity

Recombinant porcine trypsin.

No any other proteases activities contaminant.

 

 

 

 

 

 

 

 

 

Unit Definition :One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

Application

 

Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

 

Recommend Usage

 

1.Dilute recombinant modified trypsin with 50mM HAc if needed. When used, dilute it with 50mM NH4HCO3 or pH7-8 buffers directly. 1mM CaCl2 is recommended to be contained in digestion buffer. The ratio to aimed protein is 1:20 to 1:100 (w/w), the optimum pH is pH7-8.

2. When using this product, thaw it at room temperature, and mix gently before use.

3. No activity lost when freeze-thaw 5 cycles.

 

Storage Instructions

 

1. The solution should be stored under -70℃, It is stable within 24 months.

2. Above 95% activity remained after 5 times repeated freezing and thawing.

3. A 0.05 mg/ml solution of sequencing grade modified recombinant trypsin retained above 95% after a 3 hours incubation at 37 ℃ in 50mM NH4HCO3. For long-term such as 20hours incubation, 1mM CaCl2 is recommended to be contained.

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