Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

MOQ:1g
Price:450$/g
PRODUCT DESCRIPTION
High Light:

porcine trypsin

,

trypsin enzyme

Brand Name YaxinBio
Certification ISO 9001 2015
Minimum Order Quantity 1g
Model Number RPT0201
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ABOUT US
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
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Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro
MOQ: 1g
Price:450$/g
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About Us
Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
No.466 YinDu Road,XuHui, Shanghai, China ,China
5.0
Verified Supplier
Company Name Shanghai Yaxin Biotechnology Co.,Ltd.
Business Type: Manufacturer,Exporter,Seller
Brands: YAXINBIO
No. of Employees: 20~30
Year Established: 2008
Annual Sales: 50000-100000
Company Location No.466 YinDu Road,XuHui, Shanghai, China
Factory Location No.466 YinDu Road,XuHui, Shanghai, China
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile
China Shanghai Yaxin Biotechnology Co.,Ltd. manufacturer profile

       Shanghai Yaxin Biotechnology company was founded in 2008,which is a high-tech enterprise that focuses on researching and producing the recombinant proteins.YaxinBio is the first and only a company on researching and producing the recombinant carboxypeptidase B and recombinant trypsin.Animal original free enzymes are specially used in the human recombinant insulin production,and exporting abroad.

       Recombinant trypsin is used in the cell culturing process,which conforms to the 2014 USP on the usage of trypsin in the cell culture.Animal original free is very important for the production of antibody and vaccine.Usage of recombinat trypsin fundamentally solves the problem of animal original virus contamination in the human recombinant insulin production.

       YaxinBio insists on “scientific and technological innovation”,has developed with independent intellectual property rights of the products.YaxinBio obtained the support of Chinese ministry of science and technology and Shanghai innovation fund project in 2010; Recombinant trypsin,recombinant carboxypeptidase B and recombinant protein A won the Shanghai high-tech achievement transformation project in Sep.2013.

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PRODUCT DESCRIPTION
High Light:

porcine trypsin

,

trypsin enzyme

Place of Origin China
Brand Name YaxinBio
Certification ISO 9001 2015
Model Number RPT0201
Minimum Order Quantity 1g
Price 450$/g
Packaging Details ice packaging, carton
Payment Terms T/T
Supply Ability 1000g per week
Purified by HPLC
Format White lyophilized powder
Specific activity ≥3800 USP u/mg pro
Purity(RP-HPLC) NLT 70% β-trysin, NMT 20% α-trypsin
Source E. Coli

 

Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

Description:

Animal Origin Free, Expressed in E.coli, Recombinant Trypsin, 3800 u/mg pro

 

Recombinant Trypsin

 

Description

 

Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

by high pressure liquid chromatography. There are no contaminating enzyme activities such as carboxypeptidase A and chymotrypsin. No protease inhibitors such as PMSF are contained in the preparation.

Calculation

BAEE Units/ml enzyme = (ΔA253nm/min Test - ΔA253nm/min Blank)(df)
(0.001) (0.20)

 

 

 

df = Dilution factor
0.001 = The change in A253nm/minute per unit of Trypsin at pH 7.6 at 25 °C in a 3.2 ml reaction

mix 0.20 = Volume (in milliliters) of enzyme used

Units/mg solid= units/ml enzyme
mg solid/ml enzyme

 

 

 

Notes
1.This assay procedure is not to be used to assay immobilized trypsins
2.For the USP/NF procedure refer to the USP monograph
3.This procedure is for informational purposes. For a current copy of Sigma’s quality control procedure contact our Technical Service Department.

Unit Conversions
1 TAME µM Unit = 0.27 BAEE µM Units
1 BAEE µM Unit = 3.64 TAME Units
1 TAME µM Unit = 55 BAEE A253 Units
1 BAEE A253 Unit = 0.018 TAME µM Unit
1 TAME µM Unit = 180 TAME A247 Units
1 TAME A247 Unit = 0.33 BAEE Units
1 USP Unit = 3.0 BAEE Units
1 NF Unit = 1.1 USP Units

 

Advantages

 

Animal origin free: The use of recombinant Porcine Trypsin eliminates the risk of virus presence,

and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant

Porcine Trypsin belongs to the AOF level 3.

Stablility: A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

High purity:

1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.

 

Recommend Usage

 

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.

 

Storage Instructions

 

Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable

within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and

above 90% activity remained after 10 times repeated freezing and thawing.

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